General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to hom*ogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
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Specificity
Clone 1J22 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Calpain -9. It targets an epitope within 21 amino acids from the N-terminal half.
Immunogen
KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal half of human Calpain-9.
Application
Quality Control Testing
Evaluated by Western Blotting in HT-29 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Calpain-9 in HT-29 cell lysate.
Tested applications
Affinity Binding Assay: A representative lot of this antibody bound Calpain-9 with a KD of 1 x 10-12 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Calpain-9 in HT-29 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Calpain-9 in human stomach tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Calpain-9, clone 1J22 ZooMAb®, Cat. No. ZRB1383, is a recombinant Rabbit monoclonal antibody that specifically targets Calpain-9 and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Calpain-9 (UniProt: O14815; also known as Digestive tract-specific calpain, New calpain 4, nCL-4, Protein CG36) is encoded by the CAPN9 (also known as NCL4) gene (Gene ID: 10753) in human. The mammalian calpains include both ubiquitous and tissue-specific members. Two major ubiquitous calpains, m-calpain and μ-calpain, and two gastrointestinal-tract (GI) specific calpains, calpain-8 and calpain-9 are found in stomach and intestine. Calpains-9 has a typical domain structure that is similar to calpains 1 and 2. It has a regulatory N-terminal domain (domain I), a protease domain (II, consisting of sub-domains IIa and IIb), a C2-like Ca2+/phospholipid-binding domain (III), and a penta-EF-hand (PEF) domain (IV). Its catalytic region is localized in amino acids 43-337. Calpain-9 expression is down-regulated in gastric cancer tissue and in gastric cell lines of differentiated and poorly differentiated types. It is considered as a tumor suppressor and its low expression has been positively associated with late T stage (tumor size), lymph node metastasis, and advanced TNM stage. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Peng, P., et al. (2016). Sci. Rep. 6; Article 29604; Hata, S., et al. (2010). PLoS Genet. 6(7); e1001040).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
